Standard Guide for Preservation of Microorganisms

Importancia y uso:

5.1 This guide describes best practices to address characterization, storage, maintenance, and stabilization of microorganisms and the critical need for standardized and reliable methods in microbiology.

5.2 Proper preservation of microorganisms is fundamental to numerous scientific, industrial, and medical endeavors.

5.3 The significance and use of this guide is to provide a framework of best practices, equipment considerations, QC measures, and safety protocols. By outlining these considerations, this guide will increase the integrity, viability, and consistent performance of microbial stocks, thereby supporting reproducible research. While not a step-by-step protocol, this guide outlines how to develop robust, tailored preservation methods specific to the need and microorganisms of interest. Other guidelines can be consulted for more specific information on handling biological materials (6) and quality assurance (7).

5.4 Additionally, using this guide promotes awareness of the safety and QC aspects for reliable results and safe laboratory practices. In the absence of robust standardization, data could be unreliable, wasting resources, and increasing risks to human health and the environment. This guide promotes a higher standard of techniques for standardization.

Subcomité:

E35.15

Volúmen:

11.08

Palabras clave:

ambient; bacteria; cryogenic; freeze-drying; freezing; fungi; microorganisms; preservation; storage; virus;