Standard Practice for Preparing a Pseudomonas aeruginosa or Staphylococcus aureus Biofilm using the CDC Biofilm Reactor

Importancia y uso:

5.1 Bacteria that exist in biofilms are phenotypically different from suspended cells of the same genotype. Research has shown that biofilm bacteria are more difficult to kill than suspended bacteria (4, 5). Laboratory biofilms are engineered in growth reactors designed to produce a specific biofilm type. Altering system parameters will correspondingly result in a change in the biofilm. The purpose of this practice is to direct a user in the growth of a P. aeruginosa or S. aureus biofilm by clearly defining the operational parameters to grow a biofilm that can be assessed for efficacy using the Standard Test Method for Evaluating Disinfectant Efficacy Against Biofilm Grown in CDC Biofilm Reactor Using Single Tube Method (E2871).

5.2 Operating the CDC Biofilm Reactor at the conditions specified in this method generates biofilm at log densities (log₁₀ CFU per coupon) ranging from 8.0 to 9.5 for P. aeruginosa and 7.5 to 9.0 for S. aureus. These levels of biofilm are anticipated on surfaces conducive to biofilm formation such as the conditions outlined in this method.

5.2.1 To achieve an S. aureus biofilm with a population comparable to that for P. aeruginosa using the bacterial liquid growth medium conditions specified here, the S. aureus biofilm must be grown at 36 °C ± 2 °C rather than at room temperature (21 °C ± 3 °C).

Subcomité:

E35.15

Referida por:

E2756-24AE01, E2871-25

Volúmen:

11.08

Número ICS:

07.100.01 (Microbiology in general)

Palabras clave:

biofilm; coupon; growth reactor; Pseudomonas aeruginosa; reactor; Staphylococcus aureus;