Standard Test Method for Determining Disinfectant Efficacy Against Biofilm Grown in the CDC Biofilm Reactor Using the Single Tube Method

Importancia y uso:

5.1 The Single Tube Method is designed to evaluate the efficacy of disinfectants against biofilm grown in the CDC biofilm reactor following the procedures outlined in Practice E3161. Biofilm grown in the CDC reactor is representative of biofilm that forms under high fluid shear on surfaces conducive to biofilm formation.

5.1.1 Vegetative biofilm bacteria are phenotypically different from suspended planktonic cells of the same genotype. Biofilm growth reactors are engineered to produce biofilm with specific characteristics (2). Altering either the engineered system or operating conditions will modify those characteristics as well as the physicochemical environment. The goal in biofilm research and testing is to choose the growth reactor and operating conditions that generate the most relevant biofilm for the particular study.

5.2 The test method was designed to determine the log₁₀ reduction in bacteria after exposure to a disinfectant in a closed system.

5.3 The test method uses 50 mL conical tubes. The conical geometry allows for disinfectant exposure to biofilm on all surfaces of the coupon. For foaming disinfectants or for disinfectants requiring a larger volume of neutralizer, 250 mL conical tubes are used which preserve the required geometry and allow for greater neutralization capacity.

5.4 Each test includes three untreated control coupons (exposed to buffered dilution water) and five treated coupons (per disinfectant/concentration/contact time combination).

Subcomité:

E35.15

Referida por:

E2756-24AE01, E3161-25

Volúmen:

11.08

Número ICS:

71.100.35 (Chemicals for industrial and domestic desinfection purposes)

Palabras clave:

antimicrobial; biofilm; CDC Biofilm Reactor; disinfectant; efficacy testing; log reduction; Pseudomonas aeruginosa; Staphylococcus aureus;