Standard Test Method for Assessment of Fungal Biomass on Surfaces or Coatings by Measuring Fungal β-N-acetylhexosaminidase (NAHA) Activity

Importancia y uso:

5.1 This test method is used to assess fungal biomass on surface swab samples by measuring fungal NAHA activity via fluorimetry.

5.2 This test method may also be used to categorize fungal biomass loadings on a surface as being typical, atypical, or indicative of fungal growth.

5.3 This test method can assist the practitioner with a fungal assessment in buildings (Guide D7338).

5.4 The test method results can typically be obtained in less than one hour.

5.5 When analyzed in the field, this method is useful for onsite decisions regarding the presence of fungal growth or PRV when used in conjunction with visual assessments.

5.6 The test method is an objective quantitative determination of total fungal biomass.

5.7 This test method measures all the fungal structures present collectively as total fungal biomass.

5.8 This test method cannot differentiate low density fungal growth from excessive accumulation of dry (non-viable) fungal material.

5.9 There can be some variability in the enzyme activity per fungal biomass unit among fungal taxa.

Subcomité:

D22.08

Volúmen:

11.07

Palabras clave:

β-N-acetylhexosaminidase; building materials; enzyme activity; fluorescent count; fluorescent unit; fluorimetry; fluorometer; fungal biomass; fungal growth; microbiological assay; mold; NAHA;