Standard Practice for Producing High Titers of Viable and Semi-Purified Spores of Clostridium difficile using a Liquid Medium

Importancia y uso:

5.1 The quantity and quality of the spores produced by this practice may be used to assess environmental surface disinfectants for sporicidal activity (4). The method is applicable to standard as well as clinically isolated toxigenic and non-toxigenic strains of C. difficile.

Subcomité:

E35.15

Volúmen:

11.08

Número ICS:

07.100.99 (Other standards related to microbiology)

Palabras clave:

Anaerobic spore-former; Clostridium difficile; enzymatic purification; liquid medium for spore production; sporicidal activity;