Standard Test Method for Quantitative Performance Evaluation of Antimicrobial Towelettes

Importancia y uso:

5.1 The plastic Petri plate (carrier) provides a closed system for enumeration and easy application of a pre-saturated or impregnated antimicrobial towelette by an analyst.

5.2 Aliquoting of sterile 5 % non-heat-inactivated fetal bovine serum (five 10 µL spots) onto soiled carriers and inoculation of final test suspension onto treated carriers (five 10 µL spots) is conducted using a template and a positive displacement pipette, thereby ensuring a precise inoculum level and uniform distribution of soil and final test suspension.

5.3 A single towelette is tested per 2-carrier set, eliminating the likelihood of cross contamination between carriers.

5.4 The corkscrew-patterned circular motion of the product application (wipe outside to inside, wipe inside to outside using the wiping template; see Annex A3 – Annex A6) ensures uniform coverage and contact of disinfectant with the inoculated surface.

5.5 The addition of neutralizer to the treated carriers at the end of the contact time results in neutralization of the test substance. This standard test method provides a procedure for performing neutralization verification to confirm that the microbicidal, microbistatic, or both types of activity of a test substance has been reduced by 50 % at the end of the contact time (see Annex A1 for neutralization verification procedure).

5.6 The design of this standard test method minimizes any loss of viable organisms through carrier wash-off.

5.7 It is optional to adjust (dilution in PBS) the inoculum to achieve desired control counts of 5.0 log₁₀ CFU/carrier to 6.5 log₁₀ CFU/carrier.

5.8 Include, where applicable, comparisons of the test to other similar procedures such as Practices E1054 and E2362.

Subcomité:

E35.15

Volúmen:

11.08

Número ICS:

85.080.20 (Tissue paper)

Palabras clave:

antimicrobial towelettes; cell scraper; efficacy methods; membrane filtration; Pseudomonas aeruginosa; Quantitative Towelette Method (QTM); Staphylococcus aureus; test culture; wiping contaminated surfaces;