Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods

Importancia y uso:

4.1 Numerous ASTM test methods and practices (for example: Test Methods D5259 and D5392, and Practices D6974 and E2563) report colony counts as their measured parameter.

4.2 These practices provide a uniform set of counting, calculating, and reporting procedures for ASTM test methods in microbiology.

Section

A—Counting Colonies on Membrane Filters

6

B—Counting Colonies on Pour Plates

7

C—Counting Colonies on Spread Plates

8

4.3 The counting rules provide a best attainable estimate of microorganisms in the sample, since the samples cannot be held and reanalyzed at a later date.

Subcomité:

D19.24

Referida por:

E0979-20, D0932-20R24, E3363-23, F2101-25, E2196-23, E2275-24, E2169-22, E1259-23, E2563-23, E1326-25, E1891-21, D5392-24, E3321-21, E3286-21, E2647-20, E2562-22

Volúmen:

11.02

Número ICS:

07.100.20 (Microbiology of water)

Palabras clave:

calculating results; CFU; colony forming units; counting colonies; membrane filter methods; pour plate methods; reporting results; rounding off; significant figures; spread plate methods; verification of counts;