Standard Test Method for Determining Carcinogenic Potential of Virgin Base Oils in Metalworking Fluids

Importancia y uso:

5.1 The test method is based on a modification of the Ames Salmonella mutagenesis assay. As modified, there is good correlation with mouse skin-painting bioassay results for samples of raw and refined lubricating oil process streams.

5.2 Mutagenic potency in this modified assay and carcinogenicity in the skin-painting bioassay also correlate with the content of three to seven-ring PACs, which include polycyclic aromatic hydrocarbons and their heterocyclic analogs. The strength of these correlations implies that PACs are the principal mutagenic and carcinogenic species in these oils. Some of the methods that have provided evidence supporting this view are referenced in Annex A1.

Note 1: Although alkylated derivatives of three to seven-ring PACs, which include polycyclic aromatic hydrocarbons and their heterocyclic analogs were not included in the ILS, this protocol may be used to assess their mutagenicity. When testing alkylated derivatives, it is the user’s responsibility to determine the test method’s precision.

Subcomité:

E34.50

Referida por:

E2523-23, E1497-23, D6074-15R22, E2148-21

Volúmen:

11.03

Número ICS:

75.100 (Lubricants, industrial oils and related products)

Palabras clave:

base oils; dermal carcinogenicity; modified Ames test; mutagenicity;