Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water

Importancia y uso:

5.1 This test method measures the concentration of ATP present in the sample. ATP is a constituent of all living cells including bacteria and fungi. Consequently, the presence of ATP is a reliable indicator of microbial contamination in fuel systems. ATP is not associated with matter of non-biological origin.

5.2 This test method differs from Test Method D4012 as follows:

5.2.1 By providing for the rapid determination of ATP present in a fuel (petroleum) sample, a fuel and water mixture sample, fuel-associated bottom water sample, and extracellular ATP freely available in the fuel or aqueous sample matrix;

5.2.2 By providing for a method to capture, extract, and quantify ATP using self-contained test device and luminometer;

5.2.3 By providing a method of quantifying ATP present in fuel or water matrices in generally less than 10 min; and

5.2.4 By providing for the rapid separation of the ATP from chemical interferences that have previously prevented the use of ATP determinations in complex fluids containing hydrocarbons and other organic molecules.

5.3 This test method does not require the use of hazardous materials and does not generate biohazard waste.

5.4 This test method can be used to estimate viable microbial biomass, to evaluate the efficacy of antimicrobial pesticides, and to monitor microbial contamination in fuel storage and distribution systems.

Subcomité:

D02.14

Referida por:

D6974-25, D6469-24, E1259-23, D6469-24, D7847-22, D7687-23

Volúmen:

05.04

Número ICS:

07.100.99 (Other standards related to microbiology), 75.160.20 (Liquid fuels)

Palabras clave:

adenosine triphosphate; assay; ATP; ATP Bioluminescence Assay; bacteria; biocontamination; biodeterioration; biomass; capture solution; cellular; extracellular; fuel; fungi; hydrophilic particles; luciferin; luciferase; luminometer; microbe; microbiology; Relative Light Unit (RLU);