Standard Test Method for Automated Analyses of Cells—the Electrical Sensing Zone Method of Enumerating and Sizing Single Cell Suspensions

Importancia y uso:

4.1 The electrical sensing zone method (also called electrozone sensing or electrozoning) for cell counting is used in tissue culture, government research, and hospital, biomedical, and pharmaceutical laboratories for counting and sizing cells. The method may be applicable to a wide range of cell sizes and cell types, with appropriate validation (17).

4.2 The electrical sensing zone methodology was introduced in the mid-1950s (15). Since this time, there have been substantial improvements which have enhanced the operator's ease of use. Among these are the elimination of the mercury manometer, reduced size, greater automation, and availability of comprehensive statistical computer programs.

4.3 This instrumentation offers a rapid result as contrasted to the manual counting of cells using the hemocytometer standard counting chamber. The counting chamber is known to have an error of 10 to 30 %, as well as being time-consuming (16, 18). In addition, when counting and sizing porcine hepatocytes, Stegemann et al. concluded that the automated electrical sensing zone method provided greater accuracy, precision, and speed, for both counts and size, compared to the conventional microscopic or the cell mass-based method (7).

Subcomité:

F04.43

Referida por:

F2739-19, E2805-18, F3209-24A

Volúmen:

13.01

Número ICS:

07.100.01 (Microbiology in general)

Palabras clave:

automated cell counting; electrical sensing zone; electrozone sensing; electrozoning; size distribution;