Standard Test Method for Immunological Assay to Quantify Extractable Guayule Natural Rubber (GNR) Proteins

Importancia y uso:

5.1 NRL derived from Hevea contains over 200 proteins a number of which are allergens capable of eliciting Type 1 anaphylactic reactions. Guayule Natural Rubber derived from the Parthenium argentatum plant contains many fewer proteins and at much lower levels than Hevea NRL. While there have been no reports of allergic reactions to GNR proteins at present, they have been shown to induce antibody production in exposed individuals.4 An immunological assay that tests for the presence of GNR has the advantage of providing the specificity and sensitivity that other ASTM standards such as Test Methods D5712 and D6499 do not offer.

5.2 This test method describes an immunological method for the quantitation of Guayule natural rubber proteins using rabbit anti-GNR serum. Rabbits immunized with GNR proteins react to the majority of the proteins present, and their sera have the capability to detect most if not all of the proteins in GNR. This test method may be used to determine if a test item contains material derived from a natural source.

Subcomité:

D11.40

Referida por:

D7850-24

Volúmen:

09.02

Número ICS:

83.040.01 (Raw materials for rubber and plastics in general)

Palabras clave:

ELISA; GNR Guayule Natural Rubber; proteins;