Standard Test Method for Immunological Measurement of Antigenic Protein in Hevea Natural Rubber (HNR) and its Products

Importancia y uso:

5.1 Type 1 latex allergy most commonly manifests as localized urticaria after contact of skin with natural rubber but can also include symptoms of allergic rhinoconjunctivitis, asthma and rarely anaphylaxis. This immediate (Type I) allergy is caused by HNRL proteins inherent to the rubber tree, which remain on the finished products. The quantification of protein levels in HNRL products using the standard colorimetric protein assays may give spurious results due to chemical additives in the latex formulations that interfere with the assay (2,3). Furthermore, the amount of protein found in HNRL products are often below the detection limits of the standard colorimetric protein assay (4,5).

5.2 This test method describes an immunological method for quantitation of HNRL proteins using rabbit anti-HNRL serum. Rabbits immunized with HNRL proteins react to the majority of the proteins present, and their sera have the capability to detect most if not all of the proteins in HNRL. Therefore, although rabbit antibody reacts with antigenic material, this should not be considered as quantitative measure of total protein levels.

Subcomité:

D11.40

Referida por:

D3577-19R23, D3577-19R23, D7850-24, D8243-19, D8238-24, D3578-19R23, D7329-07R24, D7103-19R23, D7198-05R23, D1076-23

Volúmen:

09.02

Número ICS:

11.100 (Laboratory medicine)

Palabras clave:

ELISA; Hevea natural rubber latex; proteins;