Standard Practice for Enumeration of Non-Tuberculosis Mycobacteria in Aqueous Metalworking Fluids by Plate Count Method

Importancia y uso:

5.1 This practice allows for the recovery and enumeration of viable and culturable, non-tuberculosis, rapidly growing Mycobacteria (M. immunogenum, M. chelonae, M. absessus, M. fortuitum, and M. smegmatis) in the presence of high Gram-negative background populations in metalworking fluid field samples. This population is predominantly comprised of Gram-negative bacteria and fungi. Mycobacterial contamination of metalworking fluids has been putatively associated with hypersensitivity pneumonitis (HP) amongst metalgrinding machinists. The detection and enumeration of these organisms will aid in better understanding of occupational health-related problems and a better assessment of antimicrobial pesticide efficacy.

5.2 The measurement of viable and culturable mycobacterial densities (Guide E1326), combined with the total mycobacterial counts (including viable culturable (VC), viable nonculturable (VNC) and nonviable (NV) counts), is usually the first step in establishing any possible relationship between Mycobacteria and occupational health concerns (for example, HP).

5.3 The practice can be employed in survey studies to characterize the viable-culturable mycobacterial population densities of metalworking fluid field samples.

5.4 This practice is also applicable for establishing the mycobacterial resistance of metalworking fluid formulations by determining mycobacterium survival by means of plate count technique.

5.5 This practice can also be used to evaluate the relative efficacy of microbicides against Mycobacteria in metalworking fluids.

Subcomité:

E34.50

Referida por:

E2889-23, E2523-23, E2275-19, D5465-16R20, E2148-21

Volúmen:

11.03

Número ICS:

75.100 (Lubricants, industrial oils and related products)

Palabras clave:

acid-fast bacteria; hypersensitivity pneumonitis; metalworking fluid; non-tuberculous Mycobacteria; rapidly growing Mycobacteria;