Standard Practice for Use of Gel Filtration Columns for Cytotoxicity Reduction and Neutralization

Importancia y uso:

5.1 This practice is to be used for the removal of virucidal agents from test product-virus mixtures, or from test product-neutralizer-virus mixtures, at or after the contact period and before the inoculation of these mixtures into host systems for assay of viral infectivity.

5.2 The purpose of the practice is to reduce the concentration of the cytotoxic properties of the test product and neutralizers in order to permit the evaluation of viral infectivity at dilutions that would otherwise be toxic to the host cells.

5.3 The practice is applicable to the testing of liquid, pre-saturated towelettes, and pressurized disinfectant products, as well as handwash/rub products.

Note 3: When testing products, the ability of the solution to pass through the column must be verified prior to testing. Certain products with high viscosities are unable to pass through columns. If the product is determined to be too viscous, alternative neutralization methods should be employed.

5.4 This practice is compatible with organic soil loads, hard water, disinfectants containing organic solvents, and chemical neutralizers.

Subcomité:

E35.15

Referida por:

E1053-20, E1052-20, E2011-21, E1054-22

Volúmen:

11.08

Número ICS:

11.080.20 (Disinfectants and antiseptics), 71.100.35 (Chemicals for industrial and domestic desinfection purposes)

Palabras clave:

cytotoxicity; disinfectant; gel filtration; neutralization; tissue culture; virucidal; virucidal neutralization method ;