Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians

Importancia y uso:

5.1 An acute toxicity test is conducted to obtain information concerning the immediate effects on test organisms of a short-term exposure to a test material under specific experimental conditions. An acute toxicity test does not provide information about whether delayed effects will occur, although a post-exposure observation period, with appropriate feeding, if necessary, might provide such information. Bioavailability of the test substance may also differ between real-world exposures and laboratory exposures due to site-specific water quality conditions (see Guides E1192, E1563, and E2455).

5.2 Results of acute toxicity tests might be used to predict acute effects likely to occur on aquatic organisms in field situations as a result of exposure under comparable conditions, except that (1) motile organisms might avoid exposure when possible, and (2) toxicity to benthic organisms might be dependent on sorption or settling of the test material onto the substrate.

5.3 Results of acute tests might be used to compare the acute sensitivities of different species and the acute toxicities of different test materials, and to study the effects of various environmental factors on results of such tests.

5.4 Results of acute toxicity tests might be an important consideration when assessing the hazards of materials to aquatic organisms (see Guide E1023) or when deriving water quality criteria for aquatic organisms (3).

5.5 Results of acute toxicity tests might be useful for studying the biological availability of, and structure-activity relationships between, test materials.

5.6 Results of acute toxicity tests will depend on the temperature, composition of the dilution water, condition of the test organisms, exposure technique, and other factors.

Subcomité:

E50.47

Referida por:

E1242-23, D3978-21A, D6081-22, E1415-22, E0724-21, E1604-20, E1391-03R23, E1440-23, E1191-03AR23E01, E2122-22, E2591-22, E2455-25, E1022-22, E1241-22, E1711-20, E1193-20, E1850-04R19, E2552-23, E1192-23, E1023-23, E1218-21, E1688-19, E1366-23, E1611-21, E1706-25, E1525-02R23, E1295-22, E1563-21A, E1562-22, E1439-12R19, E1367-03R23

Volúmen:

11.09

Número ICS:

07.080 (Biology. Botany. Zoology)

Palabras clave:

acute toxicity; amphipods; amphibians; copepods; EC50; fish; flow-through test; freshwater ; fish; invertebrates; LC50; macroinvertebrates; molluscs; mysids; renewal test; saltwater; static test; test design; test materials ;