Standard Practice for In vitro production of Clostridioides difficile Spores

Importancia y uso:

5.1 This practice describes a procedure for producing spore suspensions of C. difficile ATCC 700792, C. difficile ATCC 43598, or C. difficile ATCC 43599. The spore suspensions may be used in antimicrobial efficacy testing, or other laboratory testing requiring C. difficile spores. A spore crop is considered acceptable if the titer is >8 log₁₀ spores/mL, purity of 95 %, and is resistant to 2.5M HCl after 10 min of exposure.

Subcomité:

E35.15

Volúmen:

11.08

Número ICS:

07.100.99 (Other standards related to microbiology)

Palabras clave:

C. difficile; Clostridioides; liver broth; propagation; spores; spore crop; sporulation;