Standard Guide for Fluorescence—Instrument Calibration and Qualification

Importancia y uso:

4.1 By following the general guidelines (Section 5) and instrument calibration methods (Sections 6 – 16) in this guide, users should be able to more easily conform to good laboratory and manufacturing practices (GXP) and comply with regulatory and QA/QC requirements, related to fluorescence measurements.

4.2 Each instrument parameter needing calibration (for example, wavelength, spectral responsivity) is treated in a separate section. A list of different calibration methods is given for each instrument parameter with a brief usage procedure. Precautions, achievable precision and accuracy, and other useful information are also given for each method to allow users to make a more informed decision as to which method is the best choice for their calibration needs. Additional details for each method can be found in the references given.

Subcomité:

E13.01

Referida por:

D8431-22, F3294-18, E3029-15R23

Volúmen:

03.06

Número ICS:

71.040.50 (Physicochemical methods of analysis)

Palabras clave:

anisotropy; calibration; fluorescence; fluorometer; lifetime; limit of detection; luminescence; qualification; quantum yield; reference materials; spectral correction; spectrometer; spectroscopy; validation; verification; wavelength;