Standard Test Method for Detection of Nitric Oxide Production In Vitro

Importancia y uso:

5.1 This test method is designed to evaluate nanomaterial capacity to induce nitric oxide production by macrophages.

5.2 Activated macrophages generate large quantities of NO. NO generated from activated macrophages is a cytostatic/cytotoxic agent (3-6).

5.3 The production of NO in excessive amounts leads to the generation of peroxynitrite by its spontaneous reaction with superoxide. Peroxynitrite causes tissue injury through its capability to damage lipids, proteins, and DNA (2).

5.4 NO is a proinflammatory mediator and it is an important marker for activation of inflammation (5, 6).

5.5 Testing the capacity of a nanomaterial to induce NO production in vitro helps in predicting the nanomaterial’s biocompatibility through anticipating and understanding the potential problems that might be encountered during its in vivo administration.

Subcomité:

E56.08

Volúmen:

14.02

Número ICS:

11.100.10 (In vitro diagnostic test systems)

Palabras clave:

immune response; macrophages; nanomaterial; nanoparticles; nitric oxide;