Standard Practice for Estimation of the Spectral Bandwidth of Ultraviolet-Visible Spectrophotometers

Importancia y uso:

4.1 These practices should be used by a person who develops an analytical method to ensure that the spectral bandwidths cited in the practice are actually the ones used.

Note 2: The method developer should establish the spectral bandwidths that can be used to obtain satisfactory results.

4.2 These practices should be used to determine whether a spectral bandwidth specified in a method can be realized with a given spectrophotometer and thus whether the instrument is suitable for use in this application. If accurate absorbance measurements are to be made on compounds with sharp absorption bands (natural half band widths of less than 15 nm) the spectral bandwidth of the spectrometer used should be better than ¹/_8th of the natural half band width of the compound’s absorption.

4.3 These practices allow the user of a spectrophotometer to estimate the actual spectral bandwidth of the instrument under a given set of conditions and to compare the result to the spectral bandwidth calculated from data given in the manufacturer's literature or indicated by the instrument.

Subcomité:

E13.01

Referida por:

E0275-08R22, E1401-21, D5463-18, E2056-04R16, E1164-23, D8470-22, E1026-23, E2153-01R23, E1866-97R21, E1341-16R20, E1538-17, E0169-16R22, E1205-17, E1310-22

Volúmen:

03.06

Número ICS:

17.180.30 (Optical measuring instruments)

Palabras clave:

molecular spectroscopy; ultraviolet-visible spectrophotometers; spectral bandwidth ;