Standard Quantitative Carrier Test Method to Evaluate the Bactericidal, Fungicidal, Mycobactericidal, and Sporicidal Potencies of Liquid Chemicals

Importancia y uso:

5.1 This test method is fully quantitative and it also avoids any loss of viable organisms through wash off, making it possible to produce statistically valid data using many fewer test and control carriers than other quantitative methods based on most probable numbers (MPN).

5.2 The design of the carriers makes it possible to place into each a precisely measured volume of the test suspension. The use of the threaded stir bars allows for efficient recovery of the inoculum even after its exposure for several hours to strong fixatives such as glutaraldehyde.

5.3 The membrane filtration step allows processing of the entire eluate from the test carriers and therefore the capture and subsequent detection of even low numbers of viable organisms that may be present.

5.4 This test can be performed with or without a soil load to determine the effect of such loading on microbicide performance. Consult the target regulatory agency on the need, type(s), and acceptable level(s) of soil load prior to testing. One type of soil load (Quantitative Disk Carrier Test Method E2197) to consider for this test is a mixture of three types of proteins (high molecular weight proteins, low molecular weight peptides, and mucous material) to represent the body secretions, excretions, or other extraneous substances that chemical microbicides may encounter under field conditions. It is suitable for working with the various test organisms included here. The components of the soil load are readily available and subject to much less variability than animal sera.

5.5 If distilled water or other diluent is not to be specified on the product label, the diluent for the test substance is assumed to be tap water. Since the quality of tap water varies considerably both geographically and temporally, this test method incorporates the use of water with a specified and documented level of hardness to prepare use-dilutions of test substance that require dilution in water before use. Consult the target regulatory agency regarding the use and level of water hardness prior to testing.

Subcomité:

E35.15

Referida por:

E2756-24AE01, E3152-23, E3092-18, E2614-15R20E01, E2895-19

Volúmen:

11.08

Número ICS:

71.040.50 (Physicochemical methods of analysis)

Palabras clave:

Bacillus subtilis spores; bactericides; chemical microbicides; Clostridium sporogenes spores; eluate; eluent; environmental surfaces; fungicides; germicides; log10 reductions; medical devices; membrane filtration; mycobactericides; Mycobacterium terrae; Pseudomonas aeruginosa; quantitative carrier test; soil load; sporicides; Staphylococcus aureus; standard hard water; surrogate; Trichophyton mentagrophytes conidia;