Standard Test Method for Quantification of a Bacillus subtilis Biofilm Comprised of Vegetative Cells and Spores Grown Using the Colony Biofilm Model

Importancia y uso:

5.1 Biofilm characteristics such as thickness, matrix architecture, and population are dependent on factors such as shear and nutrient availability and composition. Additionally, sporulation can occur within biofilms, including those formed by Bacillus subtilis.5 The purpose of this test method is to define the parameters to grow and enumerate a B. subtilis biofilm comprised of vegetative cells and spores embedded in extracellular polymeric substance (EPS). This type of biofilm could provide a greater challenge to antimicrobials than vegetative biofilm or spores alone. The biofilm generated using this method is suitable for efficacy testing of antimicrobials.

Subcomité:

E35.15

Referida por:

E2756-24A

Volúmen:

11.08

Número ICS:

07.100.01 (Microbiology in general)

Palabras clave:

biofilm; Bacillus subtilis; colony; membrane; sampling; spore;