Standard Practice for Testing for Cellular Responses to Particles in vitro

Importancia y uso:

4.1 This practice is to be used to help assess the biocompatibility of materials used in medical devices. It is designed to test the effect of particles released from medical devices and biomaterials on macrophages or other cells.

4.2 The appropriateness of the methods should be carefully considered by the user since not all materials or applications need to be tested by this practice.

4.3 Abbreviations: 

4.3.1 FCS (FBS)—Fetal Calf Serum (Fetal Bovine Serum)

4.3.2 FGFs—Fibroblast Growth Factors

4.3.3 HBSS—Hank’s Balanced Salt Solution

4.3.4 HEPES—A buffering salt (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)

4.3.5 IL17—Interleukin 17

4.3.6 IL18—Interleukin 18

4.3.7 IL1β—Interleukin 1 beta

4.3.8 IL6—Interleukin 6

4.3.9 IL8—Interleukin 8

4.3.10 LAL—Limulus Amebocyte Lysate

4.3.11 LPS—lipopolysaccharide (endotoxin)

4.3.12 MCP1—Monocyte Chemotactic Protein-1

4.3.13 MMPs—Matrix Metalloproteinases

4.3.14 NO—Nitric Oxide

4.3.15 PBS—Phosphate Buffered Saline

4.3.16 PGE2—Prostaglandin E2

4.3.17 RPMI 1640—Specific Growth Medium (Roswell Park Memorial Institute)

4.3.18 TGFβ—Transforming growth factor beta

4.3.19 TNFα–—Tumor Necrosis Factor alpha

4.3.20 VEGF—Vascular Endothelial Growth Factor

Subcomité:

F04.16

Referida por:

F2064-17, E2525-22, E3351-22, E3483-25, F2103-18, E3238-20, F0748-25, F1983-23, E2524-22, F3335-20, E2526-22, F2315-18, E3219-25, F1904-23

Volúmen:

13.01

Número ICS:

11.040.40 (Implants for surgery, prothetics and orthotics)

Palabras clave:

biocompatibility; cellular uptake of particles; collagenases; cytokines; growth factors; interleukins; macrophages; matrix metalloproteinases; pro-inflammatory and tissue remodeling potential; reactive oxygen/nitrogen species;